3/4/2023 0 Comments Caprine person![]() This persistent viral infection results in arthritis in adult goats and encephalitis in lambs. La frecuencia serológica indica una alta endemicidad del LvPR en rebaños de pequeños rumiantes del noreste de México.Ĭaprine arthritis-encephalitis (CAE) in goats is a complex disease syndrome caused by a lentivirus. Los factores de riesgo asociados a la presencia de anticuerpos contra el LvPR fueron presencia de animales con artritis, asistencia veterinaria, reutilizar agujas, alteraciones nerviosas, bajo índice de preñez, tipo de rebaño y mastitis. Acorde al tipo de rebaño la seropositividad en rebaños caprinos fue de 62.0 %, en ovinos de 25.4 % y de 50.2 % en rebaños mixtos. La proporción de rebaños seropositivos en general fue estimada en 50.6 %. Se aplicó un cuestionario a los productores y los datos se analizaron para determinar los factores de riesgo asociados a la seropositividad del rebaño mediante regresión logística. Las muestras se obtuvieron de animales seleccionados al azar en los años 2019 y 2020. De cada rebaño 4 a 5 muestras de suero fueron mezcladas y analizadas por ELISA para identificar anticuerpos contra la glucoproteína 135 del LvPR. De 128 rebaños, 71 de caprinos, 32 de ovinos y 25 mixtos (caprinos + ovinos), se recolectaron 768 sueros individuales de animales ≥1 año de edad. ![]() Se realizó un estudio transversal con el propósito de determinar los factores de riesgo asociados a la frecuencia serológica de Lentivirus de los pequeños rumiantes (LvPR) en ovinos y caprinos del noreste de México. Understanding the strains that circulate in the goat population will allow implementing better diagnostic methodologies to propose disease control alternatives for this emerging animal production system in Mexico. In this study, we identified a strain with high homology to Asiatic strains that manifested low virulence in kids during short infection. The virus was systemically distributed among organs the cerebellum and carpal synovial fluid samples were those with the highest viral load, 5.73 copies of virus per ng of total DNA (log10) and 5.07 log10, respectively. In the central nervous system, moderate gliosis, meningeal congestion, lymphocyte infiltration, satellitosis, and demyelination were observed. ![]() The microscopic lesions in the respiratory tract consisted of moderate interstitial pneumonia, peribronchial lymphoid hyperplasia, and bronchial exudate. At necropsy, macroscopic alterations were not observed, except in the lungs. The high viral load values persisted throughout the entire experiment (90 days), and no seroconversion was detected. The kids did not present clinical disease after the experimental infection. Virulence of the isolated strain was analyzed during an experimental infection in kids. The phylogenetic analysis revealed that the strain SRLV/B1/Goat/Mx/INIFAP-1/2013 shares a great homology with an American and Asiatic strains of the B1 genotype. From a chosen case, an SRLV strain was isolated (which was called SRLV/B1/Goat/Mx/INIFAP-1/2013). Through a real-time PCR differential test, the prevalence of genotype B was identified. Prevalence rates of 19.6% and 11.6% were detected through PCR and serology, respectively. In this study, sampling was performed to identify the percentage of SRLV positivity in different farms in Mexico. In Mexico, the B1 genotype of SRLV has been detected as endemic in goats and sheep however, its pathological characteristics have not yet been evaluated. They cause a systemic, chronic, incurable, degenerative disease. Small ruminant lentiviruses (SRLVs) are one of the main viral agents affecting goats worldwide. Because of globalization of markets and increased trading among nations, the rapid identification and reporting of diseases such as CAEV are important to prevent the dissemination of these diseases. To our knowledge this is the first report of CAEV isolation in Mexico. Previous serological surveys indicate that infection with CAEV is prevalent among goats in Mexico. One of the goats developed polyarthritis 8 mo after inoculation. These goats seroconverted to CAEV as determined by the AGID test, and CAEV was re-isolated from these goats. Subsequently, cell supernatants from the GSM cells co-cultivated with BMN cells were used to infect 2 CAEV-seronegative goats. The lentivirus was identified as CAEV by the observation of giant multinucleated cells (syncytia) in goat synovial membrane (GSM) monolayers co-cultivated with blood mononuclear (BMN) cells from the seropositive goats, and by amplifying a DNA segment of the CAEV gag gene using the polymerase chain reaction (PCR) technique. A lentivirus was isolated from 2 goats in Mexico that were seropositive to caprine arthritis encephalitis virus (CAEV) by the agar gel immunodiffusion (AGID) test. ![]()
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